FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4302104
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG17400.057.1027878
CTGTCGC20850.047.9994439
GTCTCGC16850.047.4782751
TCGCTCT18950.042.933544
TCTCGCT19650.038.8392452
CTCGCTC20500.037.9312673
GTCTTGC34850.037.8460121
CGCTCTG23100.034.5975
TTGCTCT44000.030.2723754
GTGTTAG27000.027.7614041
GTTAGCC19250.027.67763
TGTACCG2955.2386895E-1026.8471475
GTCTCAC42550.025.4075951
CTTGCTC48300.025.1922553
TTACACT17550.024.6150994
TCTTGCT50750.024.5435562
TGTTAGC24150.024.1487882
TGGACCG4800.023.9997235
GTACCGT2701.7659113E-723.9997226
GTTTCAC49800.023.8551453
Produced by FastQC (version 0.11.5)