FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3794570
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13800.037.564938
GTCTCGC16650.033.7668461
TCGCTCT16350.033.027274
TCTCGCT18700.029.6468322
CTGTCGC19000.029.178729
GTCTTGC28950.027.138641
CTCGCTC18600.027.0965673
CGCTCTG18150.025.7849255
TTACGCT2951.5399564E-824.4065914
TTGCTCT39200.023.142684
GTCTCAC34750.022.194181
GTACTAT7050.021.4704071
GTCTTAC12550.021.250451
GTATTAA10200.021.1997721
GTGTAAG18700.020.8143231
GTGTAAC10050.020.7989831
CTTGCTC36000.020.1998443
GTACTAA7200.020.0220071
GTTTCAC48600.019.85173
GTACGAC2206.1897107E-419.6362133
Produced by FastQC (version 0.11.5)