FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3794570
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC16100.035.777171
GTCTTGC28250.033.1334571
TCGCTCT17300.030.7979454
GTATTAG10950.028.9321331
CTCGCTC18900.027.8097553
TCTCGCT20550.026.978332
CGCTCTG18250.024.8550035
TCTGTCG21150.024.85168
GTGTTAG21200.023.7740971
GTCTCAC32600.023.4115491
GTACTAA7500.023.0404971
TTGCTCT40700.022.1132074
GTCTTAC12100.022.0170041
GTCCTAG14100.020.4259721
GGTTAAC6350.020.409891
CTTGCTC38200.020.3561943
GTGTAAT13850.019.754941
GTTTCAC42500.019.651933
GTATTGA11200.019.286131
GTACTAG7150.019.1332821
Produced by FastQC (version 0.11.5)