FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3744815
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC29350.036.0620541
TCGCTCT17300.032.046464
GTCTCGC15600.031.3852461
CTCGCTC17850.028.2354853
GTCTTAC12550.028.112121
TTGCTCT41150.026.595564
GTATAGG9600.026.250531
CGCTCTG17100.025.263335
TCTGTCG21250.024.3957848
CTTGCTC41050.024.2047923
TCTCGCT21050.023.9428352
GTACTAC6100.023.6070331
TTACACT18150.023.4051174
GTGTTAA16900.023.0063781
GTATTAG12250.022.922911
GTCTCAC38950.022.7372991
GTATTGC9850.022.6603551
GTACTAG7050.022.4685361
GTATTAA11850.021.8738571
GAGTGCA73350.021.7915576
Produced by FastQC (version 0.11.5)