FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3306388
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13050.045.7953038
GTCTCGC13000.038.2282071
CTGTCGC16800.034.287369
GTCTTGC28400.031.7007731
TCGCTCT14550.031.174874
TCTCGCT15700.030.7331712
CTCGCTC16300.028.718253
GTATTAC7250.026.822791
CGCTCTG16150.026.7505135
GTGTAAC10500.026.0658871
GTTTCAC42500.025.9259533
GGGTTTC45650.025.401731
GTATTAG12200.025.3855651
GTATTAA8850.025.2288021
TTGCTCT37200.024.9674324
ACCGTGT11550.024.9362648
CTTGCTC35150.023.7664783
GTGTTAG24550.023.763571
GTCTAGA14000.023.1506231
TACACCG2252.7986192E-522.4010775
Produced by FastQC (version 0.11.5)