FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3306388
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13450.043.907811
TCTGTCG14950.039.007638
TCGCTCT14950.038.5260544
CTCGCTC15150.035.166153
GTCTTGC28800.035.009491
TCTCGCT16550.032.628362
CGCTCTG16800.030.855245
CTGTCGC22250.029.1218019
GGATAAC7350.028.4158671
TTGCTCT37150.025.3873984
GTATTAA10650.025.0208681
GGGTTTC47550.024.9910011
GTTTCAC45250.024.8205683
GTCTCAC33550.024.2569471
GTATTAG12000.024.0065081
TTACACT15300.023.527964
GTACTAG7750.023.2321031
GTGCTAC10900.023.1255341
GTGTTAC13150.023.0024321
CTTGCTC37850.022.8255423
Produced by FastQC (version 0.11.5)