FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2418776
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9250.035.8183751
TCTGTCG8600.033.4914938
GTCTTGC21100.031.063381
CTCGCTC11700.028.9323563
GTATTAG8750.028.810431
GTTAGCC12250.028.809243
TCGCTCT11350.028.545384
CGCTCTG11100.028.5432035
TCTCGCT11900.027.2356262
CTGTCGC12100.026.7793589
CTCGATC10100.025.6657648
GTGTAAG11700.025.2399081
GTGTAAA14000.025.2091271
TCTCGAT9750.025.1100377
GTTTACG1450.001274755924.8365781
GTCCTAC6100.024.7958621
TCGATCT9050.024.6652859
GTGTTAC10150.024.126961
GTCTCGA20950.024.0595256
GTCTCAC25000.023.9126571
Produced by FastQC (version 0.11.5)