FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2418776
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11150.041.3261228
GTCTCGC9850.040.950561
GTCTTGC19700.035.831741
TCGCTCT10850.034.5058824
CTCGCTC11300.032.4946023
CGCTCTG11800.032.3380175
TCTCGCT12150.030.8183522
GTATTAG7600.030.328051
TACCGGT1255.370016E-428.799147
GGGTTTC32600.025.850941
TTGCTCT27850.025.593494
CTGTCGC18000.025.1992479
GTATTAA7200.025.010111
GTGTAAC6650.024.9123271
CTTGCTC25450.024.8951323
GTCTTAC8400.024.8671951
GTATAAC4251.8189894E-1223.7272381
GTCTCGA20650.023.011426
TCTTGCT31400.022.932572
TTACGGT2202.3536155E-522.9084074
Produced by FastQC (version 0.11.5)