FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4039087
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG16500.054.5496988
CTGTCGC21350.045.530479
GTCTCGC16750.043.8628431
TCGCTCT18000.039.6016044
GTCTTGC33950.038.1895181
TCTCGCT19000.036.7698552
CGCTCTG19050.035.154515
CTCGCTC20700.032.358353
GTTTCAC52300.029.1949653
GGGTTTC55700.028.5790981
TTGCTCT49650.028.2790934
GTATTAC8450.028.129931
TCTCGAT15750.027.4307027
GTCTCAC43700.027.1965581
TCGATCT16600.026.0261179
GTCTCGA35500.025.7597486
CTTGCTC45650.025.7170183
CTCGATC17500.025.5105538
GGTTTCA55050.025.2507212
TTCACCG9700.025.2406355
Produced by FastQC (version 0.11.5)