FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4039087
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC18600.046.083391
TCTGTCG18750.044.924718
TCGCTCT17900.043.8403664
GTCTTGC35600.038.4427151
CTCGCTC19600.037.833963
CGCTCTG18850.036.665755
TCTCGCT22150.035.4325032
TTGCTCT45900.028.8606324
TCTGTCA46650.027.1619978
CTGTCGC31300.027.1417839
GTGTTAG25200.026.2964841
GTCTCAC39700.026.126571
GTTTCAC50500.025.5189233
CTTGCTC44800.025.3909973
CCGTGCG4850.025.2352689
GTCTTAC14050.024.6079431
CTCGATC14650.024.571588
TCTTGCT52900.024.0916582
TTACTCT19500.023.9982434
TCTCGAT16000.023.8482537
Produced by FastQC (version 0.11.5)