FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3818760
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14700.045.558318
GTCTCGC14950.044.3263971
TCGCTCT16000.042.2995724
GTCTTGC32750.038.7094651
TCTCGCT17050.036.3277632
CTGTCGC19850.035.1895149
CTCGCTC18500.032.312533
TTGCTCT42350.030.9418374
CGCTCTG19700.028.8784755
CTTGCTC41850.027.0189723
GTCTCAC37500.024.5863761
GTGTTAG24250.024.3566681
GTCTTAC13400.024.1893121
GTGTTAC14000.024.1816331
TCTTGCT50400.023.43572
GTATAAT13400.023.1142331
CTGTCAC50650.023.0323169
TCTGTCA44750.022.6896678
GTTTCAC48650.021.6139763
GCTCTGT87200.021.0584186
Produced by FastQC (version 0.11.5)