FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4130461
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15050.038.7465678
GTCTTGC33850.031.9472221
GTCTCGC15850.029.3059771
TCGCTCT16150.028.9979554
GTGTTAC15850.027.4743541
GTACTAA8350.026.0759581
GTATAAG12100.025.7922121
TTGCTCT46200.025.7317034
GTATTAC10250.024.782761
CTGTCGC23950.024.6486439
GTATAAT11700.024.1926961
CGCTCTG19850.023.937495
GTGTAAA24250.023.6439951
TCTCGCT20050.023.387492
CTCGCTC19550.022.8786053
CTTGCTC44800.022.7052213
GTCTCAC38350.022.520971
GGGTAGT13800.022.0889841
GTATTAA12200.021.4164831
TTACTCT20250.021.3477844
Produced by FastQC (version 0.11.5)