FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4988722
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC19050.036.5931631
TCTGTCG18400.035.6048858
TCGCTCT21050.031.836254
GTCTTGC41050.031.4869441
CGCTCTG24300.027.2566155
CTCGCTC23400.027.129773
TCTCGCT25900.024.7895982
GTATTAA14050.024.290991
GTCTTAC17950.024.2722741
CTGTCGC29450.023.4677779
GTGTTAG32350.022.446541
CTGTTGC49500.021.815859
GTATAAT14100.021.629871
GTATTAC11750.021.6298681
CTTGCTC54150.021.3157583
GTCTCAC51150.021.1526281
TTGCTCT60300.021.0322824
TACACTG29100.021.0288895
GTCTCGA37700.021.005716
GTATTAG18250.020.6901761
Produced by FastQC (version 0.11.5)