FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4988722
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC40200.033.9357261
GTCTCGC19650.033.4134671
TCTGTCG23800.029.3381888
GTATTAA13550.027.4582251
TCGCTCT23300.026.5693554
GTATTAC10050.026.1323241
GTGTAAC14900.025.4600161
GTACTAT9950.024.9285741
CTCGCTC24650.023.9461363
GTCTTAC16150.023.9411451
GTGTTAG29250.023.6940521
CTTGCTC54400.022.759783
GTATAAG13400.022.3213621
CGCTCTG24550.022.2863945
TTGCTCT55150.021.6671144
GGGTTTC67150.021.6196481
TCTCGCT28900.021.4233322
GTATTAG14350.021.352021
GAGTGCA96900.021.1735886
GTACTAC8350.020.968451
Produced by FastQC (version 0.11.5)