FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3695567
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9800.025.0135921
TCTGTCG10450.024.4504768
GTAGATC20050.023.56422270
CTGTCGC12300.022.1957689
TCGCTCT12300.021.3415074
TGTCGCC12550.020.35916110
ATACCGC1055.469132E-420.0003136
TGTAGAT33500.019.84917670
CGCTCTG12350.019.837835
TCTCGCT12900.019.5433862
CTCGCTC12900.019.5410063
GTATAAG6850.019.4266131
GTATAAT6400.019.1510311
GTCTTGC21500.018.8939881
TCGCCCA13050.018.77449412
GTCGCCC12300.018.21191211
CGCCGTA2551.1314114E-917.84196768
TAATACT7250.017.3793474
GTATAGA6500.017.2401371
GTGTAGA42800.017.08977170
Produced by FastQC (version 0.11.5)