FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3950849
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA15800.029.01856270
ATCTCGT28600.028.39119370
TCTGTCG10400.025.2403348
GTACCGA1151.4972502E-624.3517841
CCTATCG2800.023.74965370
TGTCGCC11550.023.03025610
GTCTCGC12500.022.1235961
CTGTCGC12450.021.365429
TCGCTCT11500.021.0028824
TATCTCG40050.020.44913769
TCTCGCT13450.019.7796652
GTCGCCC11900.019.70584311
GCCTATG16450.019.36141870
GTACTAA4550.019.2338941
GCGTAGA1107.473356E-419.0940131
TAATACT7950.018.9334144
GTCTTGC21550.018.842971
CTCGCTC13850.017.691963
GTATAAA9550.017.5944821
TGCGAAC1000.00969454117.49996412
Produced by FastQC (version 0.11.5)