FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3874307
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9200.025.5026551
GTAGATC17000.025.32192670
TCTGTCG9900.023.3342638
TCGCTCT10950.022.6949674
CGAATAC3050.021.80189570
TGTCGCC10350.021.64365410
CTGTCGC11600.020.8197949
TGTAGAT34350.020.68282170
GTCGCCC10750.019.21031611
GCCGACC5200.018.8449670
GACGAAA7000.018.49882770
TCTCGCT11950.018.4607182
CTCGCTC11800.018.3964983
GTCTTGC23450.018.367951
GTATTGA6700.018.2932831
GTATTAT7000.018.009551
CGCCGTA2155.6053977E-817.90607368
TCGCCCA12350.017.57177712
GTGTAGA43600.017.41862170
GTGTATA8650.017.4080751
Produced by FastQC (version 0.11.5)