FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3689718
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG703.4893215E-530.0045241
TCTATCG3100.025.96743470
TCTGTCG9700.024.1762838
ATCTCGT20100.023.15893270
TCTCGTA10450.023.10977670
CTGTCGC11950.021.9665669
TGTCGCC11650.021.33022110
GTATTAG6900.021.307561
GTCTCGC9200.020.5465771
GTCGCCC11200.019.68726711
TATCTCG25300.019.36736170
GTATTAA6950.019.1395781
TAATACT6350.018.7432384
TTATACT6950.018.1324774
GTCTTGC23700.017.8718951
GTACGGT1401.864743E-417.5028763
GTATAAT6500.017.2333681
TATACTG8150.017.1807385
TCGCCCA12550.017.01175112
GTATTCG1250.001761811816.8025341
Produced by FastQC (version 0.11.5)