FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3689718
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39700.10759629868732515No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTAT2350.037.23158370
CGTTTCG1001.399396E-524.5010349
TCTGTCG8800.023.8646438
TGTCGCC9950.022.51351410
GTAGATC15950.022.16154370
GTCGCCC9900.021.56685811
CGACGAA1304.3517102E-621.53703570
CTGTCGC10400.021.202829
TCGCCCA11400.019.95725412
GACGACA5950.019.41047970
GTCTCGC10650.019.3983761
GTGTAAA13900.019.3971941
TGTAGAT28700.018.77924370
GTCTTGC21100.018.586541
GTCTTAC9150.018.36891
CTTATCG1150.001008307218.261649
TCGCTCT11000.018.1366394
GTATAAG7150.018.120021
CGGTGTA48450.017.9141970
GCCGACC4950.017.67559870
Produced by FastQC (version 0.11.5)