FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3032771
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAC2450.038.56980570
GCCGACC5050.031.18680470
ACGAGCA4450.028.31341270
CGAGCTA2850.025.78838770
TCTGTCG7200.023.3331188
ACCGCGT800.002668287121.87486
GTCTTGC18450.021.0657481
TGTCGCC8550.020.05829410
GCGTTAC1055.453953E-420.0083921
TAATACT6350.019.8423374
CTGTCGC8700.019.7124639
TATCGCA1257.833306E-519.59949519
GTCTCGC8600.019.1359351
GTAGATC11600.018.70610870
GTGTAAT9050.018.5713251
GTGTAGA27200.018.3999670
GTATTAA4950.018.3915541
ACTATCG1351.4136866E-418.14768217
GTGTTAG12900.017.9144921
TGTAGAT20500.017.75534870
Produced by FastQC (version 0.11.5)