FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3746943
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATG8300.037.5291970
TCTGTCG9050.028.6196028
TCTCGTA7350.027.61836270
TGTCGCC11050.024.38983710
CTGTCGC11350.024.3619299
ATCTCGT12550.022.86795870
GTCGCCC11000.020.68158111
GTACTAG4300.020.351321
GTATTAG7550.019.9362071
GTATTAT5950.019.4141331
GTCTTGC20700.018.9395081
TATCGTA1301.05864914E-418.84568870
GTGTTAG15650.018.3408971
GTCTCGC10900.017.9838471
TACACTG14400.017.5028365
TCGCCCA12700.017.36200512
GGTATAC5050.017.3288481
GTGTAAC7400.017.0291041
ATTAGAC6200.016.937553
TAGTCCG1452.4381529E-416.899295
Produced by FastQC (version 0.11.5)