FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3746943
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACAC3950.030.12507870
TCTGTCG9600.027.7095388
GTCTCGC11850.026.5955031
TGTCGCC11950.024.31068210
CTGTCGC11950.023.7248829
TCGCTCT12100.022.5629654
GTAGATC13750.021.88981670
GCCGACC6100.021.80218970
GTCTTGC20200.020.9757771
GTCGCCC11150.020.71866611
TCTCGCT13600.020.3385522
CGCTCTG13250.019.5474995
GTATAGG6300.018.8982851
CTCGCTC14300.018.8517533
TCGCCCA13450.018.4766412
GTATTAC6050.017.9428061
TAATACT7050.017.376644
CCGACGC4900.017.14270
GTATTAG8100.016.8602331
GTACCGT1250.001757739716.8083571
Produced by FastQC (version 0.11.5)