FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3363709
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9500.025.056661
TCTGTCG8650.022.2546048
TAGTACG952.7799245E-422.1088184
GTCGTAC1003.931535E-421.0033761
TCGCTCT11200.020.9408664
TCTCGCT11350.020.0472742
GTATAGA5200.019.5223671
TGTCGCC10500.018.66633810
TCTCGTA6800.018.52908570
GTGTTAT7000.018.5029741
GTCTTGC21000.018.0028931
CTGTCGC10500.017.9996839
ATCTCGT9950.017.93938370
GTATTAT5150.017.6727451
GTATATA7200.017.5028151
GCGCTAC1401.8647316E-417.5028131
CTCGCTC13250.017.4367663
GTATTAG7350.017.1456151
GTATAGC4300.017.0957721
GTCGCCC10250.016.73141111
Produced by FastQC (version 0.11.5)