FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3047220
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9450.027.03728
ATCTCGT15900.026.6345170
TCTCGTA6600.025.45386570
TCTCGTT4950.024.0397670
TGTCGCC11050.023.75541110
GTATTAG5450.023.124721
CTGTCGC12150.022.4689049
TAATACT4200.021.6717784
GCGTTAC1003.9294263E-421.0049531
GTCGCCC11200.020.62512611
GTATACG1055.4607185E-420.0047171
GTCTTGC17900.019.948841
GTATTAT5500.019.7319261
TCGCCCA12300.019.63426812
GTATAAG5350.019.6307981
TAGTACG1257.8175945E-519.6046224
TCGCTCT9650.019.590114
CCGACGT950.00720346218.4253984
GTATAGA5900.018.3941691
GAATAAT10300.018.3538421
Produced by FastQC (version 0.11.5)