FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3026412
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8100.027.6545728
GCGTAAC906.1628507E-627.2319131
CGACGAT906.1810842E-627.22156770
GCCGACC4600.024.34723970
CTGTCGC9900.022.6264659
GTCTCGC7900.022.6029821
TGTCGCC9600.022.23978210
CGTTTCG800.002668004521.8751969
TCGCTCT8550.021.6971764
GTAGATC11900.021.47007270
CGAATAC1205.7132536E-520.420563
GTACGAG1558.7290573E-720.3298171
GTCGCCC9250.020.05390211
TGTAGAT22350.020.0442670
GTCTTGC18050.019.979411
TCGCCCA9750.019.7434412
TATACGG900.005278543619.4446185
ATGGTCG1301.0582894E-418.8463235
GTATTAC5100.018.5360091
CTCGCTC9450.018.522053
Produced by FastQC (version 0.11.5)