FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3187416
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACA4700.038.72115770
TCTGTCG9600.030.2605578
GTCGTAC657.9483766E-426.9312323
CTGTCGC10400.026.9232049
TGTCGCC10050.026.81646710
ACGATAA2350.026.80695570
CGACGAA6700.025.59552870
TCGCTCT10950.024.9308434
GTCTCGC9750.024.0615881
GTCGCCC10200.023.33380911
GTCTTGC21800.022.6473141
TCGCCCA10900.022.47787512
GTATTCG1154.1088664E-521.3134771
CGCTCTG11100.021.1245675
TATACCG1003.9377005E-420.9984535
GTATAAG7200.020.9117341
GTAGATC11800.020.76150770
TCTCGCT11250.020.2302532
GTATTGA5650.019.8315011
CTCGCTC11900.019.7118513
Produced by FastQC (version 0.11.5)