FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3119420
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATG7350.033.80870470
CGAATCG4150.027.83064870
ATCTCGT12650.027.11396670
GCGAATA4750.025.78884770
TCTGTCG8150.024.907778
TCTCGTT5000.023.79942370
CTGTCGC10050.021.5918629
GCATACG1154.1170817E-521.307931
AATCGCG1709.335963E-820.58773469
TGTCGCC10350.020.28968810
GTATAAC3850.020.0033631
GCGAATT1055.470339E-419.99951470
TCGCTCT10200.019.9046694
GTATTAG6400.019.6908111
TCGTTAC900.00527959319.44397219
CTCGCTC9800.019.2883383
GTATTGA5500.019.094121
GTCTTGC16800.018.9615211
GTCGCCC10150.018.27571111
GTCTCGC10050.018.1124971
Produced by FastQC (version 0.11.5)