FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3842647
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA13250.036.71602670
ATCTCGT26600.030.92024870
TCTGTCG10100.028.415478
TACCCGT1358.079951E-925.9323375
ACCGATT2550.024.70523870
CTGTCGC12750.024.1565489
TGTCGCC12650.023.5174810
TATCTCG35800.022.876569
GTCGCCC11950.022.25912511
GTATATG15500.022.12845670
GTCTCGC13700.021.7204151
TCGCTCT12900.021.4394074
TCGCCCA13750.019.59974512
GTCTTGC20900.018.7603761
TCTCGCT14100.018.6216242
CTCGCTC14250.018.4256083
CGTTTCG1551.9867943E-518.064289
ATACCCG1752.7930437E-618.0044524
CGCTCTG14450.017.9283085
TAACACT10450.017.7555874
Produced by FastQC (version 0.11.5)