FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3842647
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATC17350.030.2574670
TCTGTCG8700.026.9544288
TGTAGAT35600.024.38048670
TGTCGCC11100.023.33398810
GTCTCGC12100.023.1531981
TCGCTCT11650.023.1339974
CGAACAA6200.022.57922470
CTGTCGC10900.022.4776949
GTCTTGC21250.020.5995371
GTCGCCC11250.020.53417611
TCGCCCA12150.020.16543812
TCTCGCT13300.020.0088942
GGGTACG1601.181661E-619.6983071
GTCGTAT1257.7994955E-519.610761
GTGTAGA46300.019.12406369
GTGTAAG9950.019.0054021
CTCGCTC14050.018.4387593
ATACCGG950.007212976518.421336
CGTGTTA4200.018.33384910
TACCCCG2104.2811735E-818.3328935
Produced by FastQC (version 0.11.5)