FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2765971
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAC2150.027.67379670
TCTGTCG7300.025.8902978
TGTCGCC7950.025.09422910
GTCTCGC8150.024.488161
CTGTCGC8700.023.3332319
TCGCTCT9100.023.077244
GTCTTGC17800.022.8179631
GTAGATC11550.021.21164570
TAGTCCG850.00379218920.5881465
TCTCGCT9850.019.9037882
GTCGCCC8250.019.51541711
TCGCCCA8450.019.46772212
GCGTAGA1107.4609875E-419.0984211
CTCGCTC10200.018.5333523
GCCGACC3250.018.30728170
TGTAGAT21450.018.27464770
CGCTCTG10200.017.843065
GTATAAT5900.017.8036141
GTACTAA4150.017.7178121
GTGTTAA7550.017.6228241
Produced by FastQC (version 0.11.5)