FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4001170
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA12350.028.90651570
TCTGTCG10400.028.2692268
ATCTCGT23750.025.78914670
GTCTCGC11500.025.2652851
TGTCGCC12050.023.52667210
TCGCTCT13200.022.8067324
CTGTCGC12950.022.7024159
GTCGCCC12050.021.20331611
TATCTCG31400.019.84051370
TCGCCCA14200.019.22534812
GTCTTGC20450.018.6585181
TCTCGCT14000.018.5030022
CTCGCTC13600.018.2750243
CGCTCTG12950.018.1110485
GTATTAC6850.017.8863371
TACCGTA1200.001341858617.4999967
GTTTCGC5500.017.1848221
CTCGTAT7000.016.99978470
CGGTAAC1452.4379682E-416.8995041
CAATATG15000.016.56645870