FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3604030
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA8800.030.62441870
ATCTCGT16350.027.40009170
TCTGTCG8350.026.4074158
CTGTCGC10350.021.9808679
TGTCGCC10200.021.61753710
GTATTAT7300.021.0991
TATCTCG22550.020.7978370
GTCTTGC20200.018.542341
GTATAAG7450.018.324851
GTCGCCC10900.017.9815611
TGATATG13850.017.94189670
TATACTG7350.017.6218915
TAGACCG1200.001340371617.5028255
GGATACG1803.6576694E-617.502581
TCGCTCT10650.016.763274
GTACTAA5450.016.699711
GTATTAC5050.016.6361161
GTCTCGC9500.016.2129171
GTCTTAC9200.015.9806181
CTCGCTC11400.015.6604213
Produced by FastQC (version 0.11.5)