FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4049268
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA4000.032.37341370
TCTGTCG11900.028.2363518
CGCGAAA755.589608E-528.00139210
TGTCGCC14100.025.81688910
CTGTCGC14100.025.8165669
GTCTCGC12250.025.7292291
GTCGCCC12300.025.32646411
GACGATA2800.023.74883570
TCGCTCT14100.023.3336324
TCGCCCA13850.023.24996812
GTCTTGC25450.022.4295311
GTAGATC15750.021.77670970
CTCGCTC14350.020.7391413
TCTCGCT15050.020.7086772
CGAACGA850.0037934720.58722514
GTGTAGA37550.018.17487570
TGTAGAT30350.017.52797170
GTCGCCA3002.910383E-1117.5008711
CGCTCTG15600.017.499795
CGCCGTA1401.8678443E-417.49914268
Produced by FastQC (version 0.11.5)