FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3662266
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGATA3000.037.33266470
TCTGTCG10450.027.7989228
ATCTCGT20500.026.97512670
GTCTCGC10300.026.849311
TCTCGTA11150.024.48386670
TGTCGCC12000.023.62457710
TCGCTCT11450.023.5411974
TAAGACG901.9277292E-423.3373764
CTGTCGC13150.023.1554789
TATCTCG27050.020.3138769
CTCGCTC12800.019.9643963
GTATTAT7800.019.747011
GTCGCCC11750.019.36135511
GTCTTGC22450.019.3351971
TCTCGCT13250.019.2863582
TAATACT6400.019.1439424
CGCTCTG12250.018.5746465
TCGCCCA13200.018.29512612
GTATTAG7050.017.8754371
GTCTTAC8350.017.607841
Produced by FastQC (version 0.11.5)