FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3606831
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9700.032.4748768
CTGTCGC12550.026.7734459
TGTCGCC12350.026.07339710
GTCGCCC11850.024.80993511
TCGCCCA13450.022.3790112
GTCTTGC21050.022.127341
GTCTCGC10800.021.7260481
TAATACG1154.122455E-521.3044784
GTAGATC13300.019.73641670
GCGTAAC900.00526065619.4561611
GTGTAGA31150.017.64006870
TCGCTCT12700.017.6379034
TGTAGAT25100.017.56934270
GTATTAG6600.016.9799231
GTATTGA6700.016.7264921
CGCTCTG13200.016.7044165
GTGTATA9950.016.5426251
TCTCGCT13350.016.5252022
GAGTACG1300.002282871616.1635821
TACACCG1300.002292191616.1537235
Produced by FastQC (version 0.11.5)