FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3669948
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8800.029.8293088
TCTCGTA10600.029.05597570
TCGCTCT12300.024.4753514
GTCTCGC11500.024.3519461
CTGTCGC11800.023.4316989
TGTCGCC12300.022.76404610
ATCTCGT19300.022.12387570
CGCTCTG12700.022.0506735
CTCGCTC13400.021.9437123
GTCTTGC22600.021.530191
TATCTCG25500.020.17603370
GTATTAT6600.020.1549241
GTCGCCC12450.019.95967911
TCTCGCT13950.019.5732022
AATATCG3300.019.09049870
GTATATA9350.018.7197441
TCGCCCA13900.018.38114712
GTATAAG8200.017.9298611
TTGCTCT29550.017.532344
CGTGTTA4200.017.4998610
Produced by FastQC (version 0.11.5)