FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3669948
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG552.9935714E-431.82027410
TCTGTCG9800.023.9298218
TAGTACG901.929134E-423.3348674
CTGTCGC11600.022.932239
GTAGATC17500.022.79808270
CGCCGTA1905.657057E-1022.10370668
GCGTTAT1807.239578E-921.399921
GTATTAT7200.020.9135571
TGTCGCC12050.020.33328610
GTCTTGC23450.019.7116531
GTCTCGC11500.019.1838021
GTCGCCC11750.018.46930111
GTATCGA950.007210803718.42226611
TCGCTCT11650.018.3273874
CGTTTCG1150.001008238518.2618229
TCGCCCA12650.017.98512812
CGTGTTA3900.017.05240410
TCTCGCT12800.016.9604822
CGCTCTG11900.016.765585
GTCTTAC8800.016.713161
Produced by FastQC (version 0.11.5)