FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2832410
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT18450.032.62816670
TCTCGTA9400.026.80805270
CGAACGA901.9300668E-423.33293514
TATCTCG26300.022.88934169
TATGCGC952.780133E-422.1083973
TATGTCG952.7831222E-422.10488370
TCGCTCT8350.021.3802764
TAGTACT3500.021.0029774
ACGGTAC1003.9360483E-420.9996470
GTCTCGC8900.020.845651
TCTGTCG7600.020.7236948
GTATTAG5300.019.8141291
GTATTAT6050.019.0936151
GTATAAG5750.018.2634581
CTCGCTC8450.018.2274363
CTGTCGC9850.018.1218369
GTCTTGC16100.018.0460361
CGCTCTG8750.018.0025525
GTATAGG4100.017.929371
CATATCG1602.6099839E-517.499770
Produced by FastQC (version 0.11.5)