FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3442585
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA9200.022.82553770
ATCTCGT14600.022.29398570
TATCTCG20400.022.13181970
TCTGTCG7750.020.7742988
GTCTTGC22000.019.7310961
CTGTCGC8850.019.3780639
GTATAAA10050.018.8096141
GTCTCGC9600.018.5973551
TGTCGCC9250.018.54009410
CCGTTAC950.00720539318.4246221
TCGCTCT9850.017.7704584
GTATTAG6550.017.6370071
GTATAGT5100.017.1601871
GTGTATA11100.016.3995741
TAATACT6450.016.2826984
GGTCGAT1300.002289081216.1569791
GTCTTAC9150.016.0686871
CTCGCTC10750.015.631393
TAGTACT5400.015.5590224
GGGTACG1807.145641E-515.5585721
Produced by FastQC (version 0.11.5)