FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3388367
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA14850.035.35249370
CGTTCGG400.002420446134.9989724
ATCTCGT30000.028.93247870
GTCTCGC9250.023.4663851
GTATACG1652.6575435E-923.3402211
TCTGTCG7950.022.8937598
CTGTCGC9300.021.8279579
TATCTCG41450.021.2780270
TGTCGCC9850.019.89818410
TCGCTCT10950.019.5031854
CGTTAAC900.005270843419.4496122
GTCGCCC9300.019.19326611
TATACCG1107.467136E-419.0962645
TCTCGCT10300.019.0341822
CTCGCTC10600.018.4957523
CAATATG15350.018.4685170
TTAAGCG950.007201769418.426224
TATATCG950.00721465718.4205168
AATATCG2901.8189894E-1118.10291570
GTATAAG7150.017.627581
Produced by FastQC (version 0.11.5)