FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3506073
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA9850.029.84803270
TCTGTCG8550.025.379668
ATCTCGT17700.024.1245570
GTCTCGC7800.023.7860341
TCGCTCT9050.021.6604674
TATCTCG23000.020.23934470
CTGTCGC10050.019.850399
ATCGGAC900.005279528419.44409644
TGTCGCC10750.019.20895810
CTCGCTC10400.018.1756023
GTCGCCC9900.018.0302411
GTATTAC4500.017.8918861
CGCTCTG10150.017.5886525
TCTCGCT10600.017.5026822
TTACACT11750.017.2789964
TATCGCC1452.4382601E-416.8991412
GTGTATA11050.016.4733471
GTGTAAA14450.015.9888361
GTGTACG2009.959374E-615.7526371
GTGTTAG14450.015.7465811
Produced by FastQC (version 0.11.5)