FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2617279
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATATG2750.040.72647570
CGATATA2100.026.66614270
TCTCGTA6850.025.03600570
ATCTCGT11950.024.60202670
TCTGTCG6450.024.4181258
TGTCGCC7550.023.6423710
CGTTTCG901.9297958E-423.333329
TCGCTCT6700.022.465254
CTGTCGC7700.022.2727159
CCGTACC2207.2759576E-1222.2722970
GTCTCGC7750.020.7769621
TACCCGT850.003789534820.5905845
CGCTCGA850.00379247220.5878316
TATCTCG15200.020.4930270
ATACCCG1257.824374E-519.6022384
GTATAAG6200.019.1961041
GTCTTGC14500.019.0715071
TCGCCCA8100.019.01233512
CTCGCTC8000.018.8146463
CGCTCTG7350.018.5735475
Produced by FastQC (version 0.11.5)