FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2617279
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC4400.028.6353870
TCTGTCG6450.026.589258
CTGTCGC6700.024.5518659
TGTCGCC7300.023.01334610
GTCTCGC6800.022.1413211
TCGCTCT7150.022.0284794
GACGACC1755.2623363E-921.99924370
GTCGCCC7150.021.53813211
CCGACGC1154.123189E-521.30361670
GTCTTGC15000.021.2419381
TCGCCCA8450.019.46715712
CGCTCTG8050.019.1305085
TCGATAA1651.5955575E-619.08988813
GTGTAAC4250.018.9488531
ATTACGC950.007212843718.42112249
TTCGATA1551.9864383E-518.06424112
GTAGATC10500.017.9993870
CTCGCTC8150.017.6118053
GCGTTAG1401.8610523E-417.507091
GTCGACG1401.8673173E-417.499466
Produced by FastQC (version 0.11.5)