FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3725334
Sequences flagged as poor quality0
Sequence length150
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13300.040.593818
GTCTCGC15000.036.2848971
GTCTTGC29200.033.3025781
TCGCTCT16250.031.0095084
GTCTTAC10150.030.7438551
TCTCGCT17550.029.5483322
CTCGCTC17000.027.9472683
CGCTCTG17000.027.1007465
GTCTCAC32350.026.4705921
GGAGTGC72400.026.4480275
TTGCTCT38250.025.406954
GAGTGCA80100.025.07396
CTGTCGC20250.024.1731999
CTGGAGT84400.023.3696633
GGCTGGA96950.023.204371
CTTGCTC37050.022.5379353
GTACAGA13850.022.5306971
GGCTAGA17250.022.2968061
GGGTTTC44050.022.240461
GTTTCAC42300.021.4424573
Produced by FastQC (version 0.11.5)