FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3233673
Sequences flagged as poor quality0
Sequence length150
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12900.044.085348
GTCTCGC12050.042.1712071
TCGCTCT14400.036.493644
CTCGCTC14500.034.2560963
TCTCGCT15150.032.8000562
CGCTCTG13850.032.225435
CTGTCGC19100.030.9056079
GTCTTGC24800.029.5647851
GTCTCAC28650.028.1257041
TTGCTCT33300.027.2384954
CTCGATC11800.026.2327168
TCGATCT12350.025.6473529
GCCGAAC1701.08295906E-425.4073358
GGGTTTC43900.025.1353021
GGAGTGC67150.024.8713175
GTATTAA10800.024.8704131
TCTCGAT12600.024.5671467
GAGTGCA71550.024.3479566
CTGGAGT76400.024.2156733
GGCTGGA90750.024.1582411
Produced by FastQC (version 0.11.5)