FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3233673
Sequences flagged as poor quality0
Sequence length150
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13350.039.224121
TCTGTCG12900.038.5034078
TCGCTCT15100.035.7539564
GTCTTGC22500.034.7455751
CTCGCTC15000.031.193343
TCTCGCT17000.030.4904382
CGCTCTG15250.029.7379115
CTGTCGC19600.026.4433339
GGAGTGC64100.026.0537195
GTATTAA9100.025.9349081
GTACTAC4850.025.8513551
CTTGTCG3900.025.8406379
GAGTGCA70050.025.3821586
GTGTTAC9650.025.2210251
ACCATGT31600.024.8301438
TTGCTCT33400.024.7851284
GTCTCGA26500.024.719256
CTGGAGT75100.024.6338883
GTCTCAC29150.023.7829341
GGCTGGA85600.023.7799781
Produced by FastQC (version 0.11.5)