FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3556994
Sequences flagged as poor quality0
Sequence length150
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG16000.044.5408558
TCGCTCT15300.036.698354
GTCTTGC25650.036.196181
GTCTCGC14700.034.5868721
CTGTCGC22600.031.5333529
CTCGCTC17550.028.7120573
TTGCTCT32900.028.6628074
CGCTCTG16350.028.6179815
TCTCGCT18000.027.9958272
GTACAGA12850.026.3775351
GTCTCAC30250.025.8202251
GTATAAG9850.024.6863691
GAGTGCA77400.024.6461036
GGCTGGA97550.024.5491031
GGAGTGC72100.024.2613165
CTGGAGT81950.024.2439583
CTTGCTC33400.023.9232333
CTGTTGC30550.023.3274549
GTACTGG12650.022.7171381
TCTGTCA31750.022.219068
Produced by FastQC (version 0.11.5)