FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3470132
Sequences flagged as poor quality0
Sequence length150
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13950.033.542178
GTCTTGC25050.031.275911
TCGCTCT13750.027.7475834
GTCTCGC12050.027.6926611
CTCGCTC14300.026.6803683
TACACCG2456.907794E-826.4440785
GTCTCGA27850.024.8140726
GTCTCAC29900.024.747021
TCGAACT17000.024.5601529
GGTCTCG28450.024.2907525
GTATTAA10050.023.8200021
TCTCGCT14300.023.6708642
TTGCTCT35050.023.6190174
CGCTCTG15050.022.95925
GTGTAAA17500.022.7991451
GAGTGCA71600.022.7220466
GAGTAAC8650.022.6433981
TCACTCT33700.022.6426874
GGCTGGA89350.022.5706541
GGAGTGC68400.022.4168995
Produced by FastQC (version 0.11.5)