FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3470132
Sequences flagged as poor quality0
Sequence length150
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC23600.033.088691
GTCTCGC12500.032.414431
TCTGTCG14950.030.8156918
TCGCTCT13650.029.5317024
CTCGCTC15200.024.6259443
CGCTCTG14800.023.8323845
GTCCTAC7200.023.5332031
GTCTAAG8250.023.2169481
TCTCGCT17700.023.1844732
CTGTCGC19300.023.1245759
GAGTGCA68200.023.1149446
TCGAACT18000.022.795119
GTCTCGA25600.022.7760376
GGGCATG31700.022.54231
TTGCTCT34600.022.2608014
GTCTCAC27050.022.0598961
GGAGTGC66750.021.8916175
GTCTTAC9500.021.7130171
GTCCAAC6450.021.7009931
GTGCTAC8950.021.4010981
Produced by FastQC (version 0.11.5)