FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3238853
Sequences flagged as poor quality0
Sequence length150
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11950.048.1925438
GTCTCGC11750.043.8434071
TCGCTCT12700.037.4108854
GTCTTGC24700.035.5444871
CTGTCGC17950.034.088849
TCTCGCT13350.032.9115072
CTCGCTC13600.032.817883
CGCTCTG14700.028.4032735
TCTCGAT10850.026.5392137
CTCGATC10250.025.9857718
TTGCTCT34600.025.7990284
TTACACT13000.025.4725384
CTTGCTC30350.025.3794863
CCGCATA1801.5131127E-424.1859171
GTACTAC5200.023.7208021
TCGATCT11250.023.0360369
TACACTG17250.022.9525725
GTGTAAA17950.022.232181
GTCTCGA25600.022.2149266
GTCTTAC10800.022.1704241
Produced by FastQC (version 0.11.5)